Adaptation of Babson's Method for the Determination of Serum Glutamic Oxalacetic Transaminase in the Clinical Laboratory.
نویسندگان
چکیده
E NEED for an accurate and simple determination for serum glutamic oxalacetic transaminase (SGO-T) in the clinical laboratory led! us to investigate a procedure published recelltiy by Babson et al. (1,2). III this niethod the (hiazonium salt, (i-bellzamido-4-methoxv-ln-tolui(line (liazoluunl chloride, pro(111c’s a higilly specific color for the oxalacetic acid! fornied as a product of transamination. rllhe procedure is not well suited for tile routine laboratory without the use of costly automatic, devices, since the filial color formed is unstable 1111(1 increases with time. Our present work introduces tile use of sodium metabisulfite to i)i oduce a stable filial color, and presents further lno(hifications to increase accuracy. Reagents and Materials
منابع مشابه
Automated Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminase.
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3. Jacobs, W. L. W., Gamrna-glutamyl transpeptidase in disease of the liver, cardiovascularsystem and diabetesmellitus.Clin. Chim. Acta 38,419(1972). 4. Karmen, A., A note on the spectrophotometric assay of glutamic oxalacetic transaminase in human blood serum. J. Clin. Invest. 34, 131 (1955). 5. Wroblewski, F., and La Due, J. S., Serum glutamic pyruvic transaminase in cardiac and hepatic disea...
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عنوان ژورنال:
- Clinical chemistry
دوره 11 شماره
صفحات -
تاریخ انتشار 1965